|
KMID : 0895820130230010025
|
|
Journal of Oriental Rehabilitation Medicine
2013 Volume.23 No. 1 p.25 ~ p.49
|
|
|
The Effects of Bangkibokryeong-tang on Papain-induced OsteoArthritis C57BL/10 Mouse
|
|
Park In-Sun
Yoon Il-Ji
Oh Min-Seok
|
|
|
Abstract
|
|
|
Objectives：This study was carried out to know the anti-osteroarthritic effects of Bangkibokryeong-tang(Fanjifuling-tang(BBT)) on the papain-induced osteoarthritis C57BL/10 mouse．
Methods：Osteoarthritis was induced by injection of papain(6 μl) into knee joint of mouse. Osteoarthritic mice were divided into 4 groups(normal, control, joinsⓡ BBT)．The injection did not fit the normal group．A week later, after the injection of papain, control group was taken normal saline 200 μl, positive control group was taken joinsⓡ(100 mg/kg), treated group was taken extract of Bangkibokryeong-tang(Fanjifuling-tang(BBT))(400 mg/kg)．After then, we examined hepatotoxicity, nephrotoxicity, inflammation cytokines, expression of inflammation factor mRNA, hemotology, histology through the micro CT-arthrography, and etc．
Results：1．Hepatotoxicity and nephrotoxicity have not expressed．2．The levels of IL-1β, TNF-α, IL-6, MCP-1, Thromboxane B2, Leukotriene B4, Prostaglandin E2 in serum were significantly decreased．3．In hematology, the levels of neutrophils and monocytes were significantly decreased．4．The expression of inflammation factor mRNA like TNF-α and IL-6, COX-2, iNOS-Ⅱ were significantly inhibited．5．In micro CT-arthrography, cartilage volume was less decreased．6．The degree of osteoarthritis induced damage of joint of BBT group is low in histopathologic observation(hematoxylin&eosin(H&E), Safranin-O)．
Conclusions：According to this study, BBT has effect of anti-osteoarthritis．Further clinical research for the cartilage protective effect is necessary．
|
|
|
KEYWORD
|
|
|
Osteoarthritis, Anti-inflammation, Bangkibokryeong-tang, Papain, Proteoglycan
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|